Mohd Shariq
Quantlase Lab LLC,UAE
Summary
With a wealth of expertise spanning molecular microbiology, molecular immunology, molecular biology, and cell biology, I bring a multifaceted skill set and a profound commitment to unraveling the complexities of infectious diseases. My primary focus lies in understanding the intricate biology of Mycobacterium tuberculosis, an exceptionally virulent pathogen responsible for significant global morbidity and mortality. My research endeavors have delved deep into deciphering the host-pathogen interactions of Mycobacterium tuberculosis, shedding light on the underlying molecular mechanisms of its pathogenesis.
In addition to my work on Mycobacterium tuberculosis, I have extensively investigated Helicobacter pylori, a pivotal gastric pathogen implicated in diverse pathologies, including gastritis, lymphoma, and cancer. My research in this domain aims to uncover the nuanced host-pathogen dynamics governing H. pylori infections, contributing valuable insights to our understanding of the associated diseases.
Furthermore, my research portfolio encompasses a comprehensive exploration of drug resistance mechanisms in Candida albicans. My investigations have provided a detailed understanding of the molecular basis of drug resistance in this opportunistic fungal pathogen, particularly focusing on mechanisms involving efflux pumps and transcription factors.
This diverse background in molecular microbiology and immunology, coupled with a specific interest in host-pathogen interactions, uniquely positions me to contribute meaningfully to the broader field of infectious diseases research. My dedication to deciphering the molecular intricacies of deadly pathogens and drug-resistant microorganisms reflects a commitment to advancing our understanding of infectious diseases for the development of targeted therapeutic interventions.
Overview
10
10
years of professional experience
10
10
years of post-secondary education
1
1
Certification
Work History
Senior Scientist
Quantlase Imaging Laboratory
Abu Dhabi
01.2022 - 12.2023
In my capacity as a Scientist and Group Leader at QuantLase Lab LLC, I undertook a pivotal role in advancing the laboratory's capabilities and spearheading innovative research initiatives. A cornerstone of my contributions was the establishment of a BSLII laboratory, providing a controlled and secure environment for the execution of complex experiments.
Central to our research focus was the initiation of groundbreaking work in the realm of CRISPR-based diagnostics, specifically directed towards the detection of the influenza virus. Leveraging the precision and versatility of CRISPR technology, our research aimed to develop highly sensitive and specific diagnostic tools for the rapid identification of influenza pathogens. As the group leader, I orchestrated collaborative efforts among interdisciplinary team members, fostering an environment conducive to scientific exploration and innovation.
This endeavor not only demanded a keen understanding of molecular biology techniques but also necessitated effective project management and leadership skills. The establishment of the BSLII laboratory served as a testament to my commitment to stringent safety protocols and ethical research practices.
Through this research initiative, I actively contributed to the scientific community's ongoing efforts to enhance diagnostic capabilities, especially in the context of infectious diseases. The experience at QuantLase Lab LLC not only solidified my technical expertise but also honed my leadership skills in orchestrating impactful research endeavors at the intersection of cutting-edge technology and public health.
Research Associate
National Institute of Pathology
New Delhi, INDIA
03.2021 - 02.2022
In my capacity as a Research Associate at NIOP, I played an instrumental role in the investigation of the project titled "Role of Mycobacterium tuberculosis effector proteins RipA and MoxR1 in Xenophagy: Unravelling a new era in mycobacterial virulence." This research initiative was designed to unravel the intricate mechanisms employed by Mycobacterium tuberculosis (M. tb) effector proteins RipA and MoxR1 in modulating autophagy and discerning their consequential impacts on virulence and pathogenesis.
My primary focus involved a comprehensive exploration of the regulatory roles played by RipA and MoxR1 in the autophagic processes implicated in the host response to M. tb infection. The intricate interplay between these effector proteins and cellular autophagy mechanisms was meticulously dissected to unveil novel insights into mycobacterial virulence strategies. Leveraging advanced molecular biology and cellular techniques, I conducted experiments aimed at elucidating the molecular pathways through which RipA and MoxR1 influence autophagic responses.
This research not only demanded a nuanced understanding of the molecular intricacies of host-pathogen interactions but also required proficiency in techniques such as gene expression analysis, cellular imaging, and functional assays. The project's overarching goal was to contribute to the broader understanding of M. tb virulence, with a specific focus on the modulation of autophagy as a pivotal aspect of the bacterium's pathogenicity.
My experience at NIOP afforded me a rich academic and research environment, providing me with the opportunity to contribute meaningfully to the elucidation of molecular mechanisms underlying mycobacterial infections. The findings from this project have the potential to shape our understanding of host defense mechanisms against M. tb and contribute to the development of novel therapeutic strategies to combat tuberculosis.
National Postdoctoral fellow
National Institute of Pathology
04.2018 - 10.2020
During my tenure as a National Postdoctoral Fellow at the National Institute of Pathology (NIOP), Indian Council of Medical Research (ICMR) in New Delhi, I spearheaded a pivotal research project titled "Role of RipA in inflammation and antimicrobial defense through autophagy." My primary objective within this project was to meticulously unravel the mechanistic intricacies governing the role of RipA in the orchestration of inflammation and its regulatory impact on autophagy. This undertaking was undertaken with the overarching goal of advancing our comprehension of the pathomechanisms underlying tuberculosis (TB) disease.
Engaging in this research initiative, I conducted a thorough exploration of RipA's involvement in inflammation, meticulously dissecting its influence on key signaling pathways. Simultaneously, I delved into the regulation of autophagy by RipA, aiming to elucidate the molecular events through which this effector contributes to the host's antimicrobial defense strategies.
The project demanded a comprehensive approach, integrating advanced molecular and cellular biology techniques. Through gene expression analyses, cellular imaging, and functional assays, I systematically investigated the intricate interplay between RipA, inflammation, and autophagy regulation. The outcomes of this research not only contributed to our understanding of TB pathogenesis at a molecular level but also shed light on potential therapeutic targets for mitigating the impact of this infectious disease.
My experience as a National Postdoctoral Fellow at NIOP, ICMR, New Delhi, not only enriched my understanding of host-pathogen interactions but also instilled a profound appreciation for the significance of mechanistic exploration in unraveling the complexities of infectious diseases. This research endeavor serves as a testament to my commitment to advancing scientific knowledge, particularly in the context of elucidating the intricacies of TB pathogenesis.
Research Associate
IIT, DELHI
DELHI, INDIA
05.2017 - 03.2018
During my tenure as a Research Associate at the esteemed Indian Institute of Technology (IIT Delhi), I played a pivotal role in the project titled "Development of an affordable, automated, and field-deployable, point-of-care and contained system for rapid diagnosis of TB caused by Mycobacterium tuberculosis." My primary focus within this ambitious endeavor was the conception and realization of a point-of-care diagnostic device tailored for the expeditious and accurate detection of Mycobacterium tuberculosis.
The project demanded an interdisciplinary approach, amalgamating principles from molecular diagnostics, engineering, and automation. Engaging with a diverse team of experts, I actively contributed to the design, optimization, and implementation of a diagnostic platform that aligned with the project's overarching goals of affordability, automation, and deployability in resource-constrained settings.
My responsibilities extended to the fine-tuning of diagnostic assays, integration of microfluidic technologies, and the incorporation of automation elements to facilitate seamless and rapid diagnosis. The development process required meticulous attention to detail and a keen understanding of the unique challenges associated with field deployment.
This research experience at IIT Delhi not only enriched my technical proficiency in molecular biology and engineering but also underscored the importance of translating scientific innovation into tangible solutions with real-world impact. The collaborative and dynamic nature of the project fostered an environment conducive to transformative research, and the resulting point-of-care diagnostic device represents a significant stride towards addressing the pressing global health challenge of tuberculosis. This academic endeavor has not only contributed to the advancement of diagnostic technologies but also reinforced my commitment to impactful research at the intersection of science and healthcare.
Dr. DS KOTHARI Postdoctoral Fellow
School of Life Sciences
Delhi, INDIA
02.2014 - 02.2017
As a Kothari Postdoctoral Fellow at the School of Life Sciences, my research focused on unraveling the intricate mechanistic details governing drug resistance in Candida albicans at the transcriptional level, with a particular emphasis on the regulation of efflux pump overexpression. This research initiative aimed to contribute to our understanding of the molecular events underpinning the adaptive responses of Candida albicans to antifungal agents, ultimately leading to the development of drug resistance.
My investigation involved a comprehensive exploration of the transcriptional regulatory landscape associated with efflux pump overexpression in Candida albicans. Leveraging advanced molecular biology techniques, I meticulously dissected the roles of specific transcription factors and signaling pathways implicated in the upregulation of efflux pumps. The research methodology integrated genomics and bioinformatics analyses to discern the intricate regulatory networks orchestrating the transcriptional responses to antifungal stress.
The multidisciplinary nature of this project allowed for a holistic understanding of the interplay between transcriptional regulation and drug resistance mechanisms in Candida albicans. Through rigorous experimentation and systematic data analysis, the aim was to provide novel insights that could inform strategies for combating antifungal resistance.
This academic experience significantly enriched my expertise in fungal molecular biology and contributed to the broader discourse on antifungal drug resistance. The findings from this research endeavor not only advance our fundamental knowledge of Candida albicans biology but also hold implications for the development of targeted therapeutic interventions aimed at mitigating the emergence of drug resistance in this clinically relevant fungal pathogen.
Education
Ph.D. - Biochemical characterization of outer and inner membrane complexes of Helicobacter pylori Cag-T4SS
Special Center for Molecular Medicine
JNU, NEW DELHI, INDIA 08.2008 - 01.2014
M.Sc. - BIOTECHNOLOGY
Jamia Hamdard
DELHI, INDIA 07.2006 - 05.2008
B.Sc - Biotechnology
Jamia Milia Islamia
DELHI, INDIA 08.2003 - 05.2006
Skills
- HIGHLIGHTS /EXPERIMENTAL SKILLS:
- Throughout my academic and research trajectories, I have developed a robust skill set in molecular biology, molecular genetics, and protein purification techniques My proficiency extends to fundamental procedures, such as plasmid isolation, genomic DNA isolation, total RNA isolation, cDNA preparation, PCR, cloning, ligation, transformation, electroporation, transfection, and restriction digestion, with a specific focus on Mycobacterium tuberculosis (MTB) Additionally, I am well versed in advanced techniques including PCR, qRT-PCR, gene analysis, SDS-PAGE, Western blotting, 2D gel electrophoresis, and sequencing
- In the realm of molecular genetics, I have demonstrated my expertise in protein purification methodologies, including salt fractionation, gel filtration, and affinity purification of MBP, GST, and His-tagged proteins My skill set further encompasses enzyme kinetics, western blot analysis, RNA isolation, RT-PCR, site-directed mutagenesis, mammalian cell culture, animal handling, antibody generation (Mice and Rabbit), chromatin immunoprecipitation, Helicobacter pylori culture, mutant generation and complementation, pull-down assays, co-expression, and molecular and cellular immunology
- Moreover, I possess hands-on experience in employing fluorescence techniques and utilizing spectroscopic tools such as UV-Vis, fluorimeter, confocal microscopy, AFM, and TEM Complementing my laboratory skills, I am proficient in various computer applications, including statistical packages, image processors (Adobe Photoshop), Image J, basic bioinformatics tools, and online database search tools This comprehensive skill set underscores my commitment to employing cutting-edge methodologies to advance molecular and cellular research
Accomplishments
- During my academic and research pursuits, I have gained a wealth of expertise in Genetic Engineering (Recombinant DNA Technology), Molecular Biology, Molecular Cell Biology, Cell Signaling, Biochemistry, Microbiology, Host-Pathogen Interaction, and Molecular and Cellular Immunology
- My comprehensive understanding of these disciplines is underpinned by a rigorous exploration of the molecular intricacies governing cellular functions and host-pathogen dynamics
- My research endeavors have particularly focused on employing advanced molecular and immunological techniques to unravel the complexities of cellular processes
- Through in-depth studies in genetic engineering, Recombinant DNA Technology has been proficiently utilized to manipulate genetic material, thereby elucidating the fundamental principles of gene expression and regulation
- Molecular and Cellular Immunology investigations have further fortified my skills, enabling a nuanced understanding of immune responses at the molecular level
- A key facet of my research portfolio involves the study of host-pathogen interactions in microbiology
- This exploration has not only deepened our understanding of disease mechanisms but has also provided a foundation for the application of gained insights in the design of diagnostic tools
- My accrued expertise positions me adeptly in contributing to the development of diagnostic strategies tailored for the detection of emerging pathogenic organisms, thereby bridging the gap between theoretical knowledge and practical applications in the realm of infectious disease diagnostics
- Qualified CSIR- NET JRF December 2008 and June 2009 jointly conducted by the Council for Scientific and Industrial Research (CSIR) and University Grant Commission (UGC)
- Qualified CSIR- NET SRF August 2010 jointly conducted by the Council for Scientific and Industrial Research (CSIR) and University Grant Commission (UGC)
- Qualified DBT-JRF in Category A with all INDIA rank 12 in May 2008 conducted by Department of Biotechnology
- Qualified GATE with all INDIA rank 161 and 98.7 percentile in March 2008
- Obtained a good grade with a CGPA of 7.7/9 (2nd Rank) in my pre-PhD course work from Jawaharlal Nehru University
- RESEARCH PUBLICATIONS: 2023
1. Mycobacterium tuberculosis protein MoxR1 enhances virulence by inhibiting host cell death pathways and disrupting cellular bioenergetics. Virulence. Journal article DOI: 10.1080/21505594.2023.2180230 PMID: 36799069.
2. Regulation of autophagy by SARS‐CoV‐2: The multifunctional contributions of ORF3a. Journal of Medical Virology. Journal article DOI 10.1002/jmv.28959 PMID 37485696.
3. Point‐of‐care optical devices in clinical imaging and screening: A review on the state of the art. Journal of Biophotonics. Journal article DOI 10.1002/jbio.202200386 PMID 36906735.
4. Rv2231c, a unique histidinol phosphate aminotransferase from Mycobacterium tuberculosis, supports virulence by inhibiting host-directed defense. Cellular and Molecular Life Sciences. DOI 10.21203/rs.3.rs-3073689/v1, Under Revision 2.
5. CRISPR‑based diagnostic approaches: Implications for rapid management of future pandemics. Molecular Biology Reports. DOI 10.3892/mmr.2023.13005 PMID 37144477.
6. Multipronged regulation of autophagy and apoptosis: Emerging role of TRIM proteins. Cellular and Molecular Biology letters. Journal article. Accepted.
7. PRKAA2, MTOR, and TFEB in the regulation of lysosomal damage response and autophagy. Journal of Molecular Medicine. Journal article. Under revision 2.
8. Activation of the lysosomal damage response and selective autophagy: The coordinated actions of galectins, TRIM proteins, and CGAS-STING1 in providing immunity against Mycobacterium tuberculosis. Critical Reviews in Microbiology. Journal Article. Under revision 2.
9. Functional Interplay of PE18 and PPE26 Proteins of Mycobacterium tuberculosis: Impact on TH1 Immune Response and Endosome-Phagosome Maturation. Microbes and Infection. Journal article. Under review.
10. 2022
11. 10. COVID-19 and tuberculosis: the double whammy of respiratory pathogens. European Respiratory Review. Journal article DOI: 10.1183/16000617.0264-2021 PMID: 35418488.
12. The exploitation of host autophagy and ubiquitin machinery by Mycobacterium tuberculosis in shaping immune responses and host defense during infection. Autophagy 2022-01 | Journal article DOI: 10.1080/15548627.2021.2021495 PMID: 35000542.
13. ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator. International Journal of medical microbiology: IJMM 2021-12 | Journal article DOI: 10.1016/j.ijmm.2021.151544 PMID: 34922100.
14. 2021
15. Mycobacterium tuberculosis Protein PE6 (Rv0335c), a Novel TLR4 Agonist, Evokes an Inflammatory Response and Modulates the Cell Death Pathways in Macrophages to Enhance Intracellular Survival. Frontiers in immunology 2021-07 | Journal article DOI: 10.3389/fimmu.2021.696491 PMID: 34322125.
16. Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic Repurposing, and Immune Modulation. Frontiers in immunology 2021-03 | Journal article DOI: 10.3389/fimmu.2021.636644 PMID: 33746976. Teleological cooption of Mycobacterium tuberculosis PE/PPE proteins as porins: Role in molecular immigration and emigration. International journal of medical microbiology: IJMM 2021-03 | Journal article DOI: 10.1016/j.ijmm.2021.151495 PMID: 33730677.
17. Emerging Transcriptomic Approaches to Decipher Mycobacterial Complexities. Integrated Omics Approaches to Infectious Diseases 2021 | Book chapter DOI: 10.1007/978-981-16-0691-5_7 Part of ISBN: 9789811606908 Part of ISBN: 9789811606915.
18. Exploration of the Mycobacterial Proteome in the Pathogenesis of TB: A Perspective. Integrated Omics Approaches to Infectious Diseases 2021 | Book chapter DOI: 10.1007/978-981-16-0691-5_17 Part of ISBN: 9789811606908 Part of ISBN: 9789811606915.
19. Post-translational modifications in tuberculosis: ubiquitination paradox. Autophagy 2020-11 | Journal article DOI: 10.1080/15548627.2020.1850009 PMID: 33190592.
20. 2020
21. Polypharmacological Drug Repurposing Approach to Target Mycobacterium Tuberculosis. Proceedings of International Conference on Drug Discovery (ICDD) 2020-10-02 | Conference paper.
22. ESX5 associated PE proteins of Mycobacterium tuberculosis drives Th1 type polarization: an immunomodulatory paradigm. The Journal of Immunology 2020 | Journal article WOSUID: WOS:000589972400477.
23. 2019
24. CagW, a VirB6 homolog interacts with Cag-type IV secretion system substrate CagA in Helicobacter pylori. Biochemical and biophysical research communications 2019-06 | Journal article DOI: 10.1016/j.bbrc.2019.06.013 PMID: 31182283.
25. Intrinsically Disordered Regions in PE/PPE Protein Family of Mycobacterium tuberculosis: Moonlighting Function. Mycobacterium Tuberculosis: Molecular Infection Biology, Pathogenesis, Diagnostics, and New Interventions 2019 | Book chapter DOI: 10.1007/978-981-32-9413-4_9 Part of ISBN: 9789813294127 Part of ISBN: 9789813294134.
26. Mycobacterium Tuberculosis: Molecular Infection Biology, Pathogenesis, Diagnostics and New Interventions 2019 I Book DOI: 10.1007/978-981-32-9413-4 Part of ISBN: 9789813294127 Part of ISBN: 9789813294134 and 978-981-32-9415-8.
27. 2018
28. Identification of genome-wide binding sites of heat shock factor 1, Hsf1, under basal conditions in the human pathogenic yeast, Candida albicans. AMB Express 2018-07 | Journal article DOI: 10.1186/s13568-018-0647-7 Part of ISSN: 2191-0855. PMID: 30014253.
29. 2017
30. Analyzing the role of CagV, a VirB8 homolog of the type IV secretion system of Helicobacter pylori. FEBS Open Bio 2017-05 | Journal article DOI: 10.1002/2211-5463.12225 Part of ISSN: 2211-5463 PMID: 28680806.
31. The global regulator Ncb2 escapes from the core promoter and impacts transcription in response to drug stress in Candida albicans. Scientific Reports 2017-04-06 | Journal article DOI: 10.1038/srep46084 Part of ISSN: 2045-2322 PMID: 28383050.
32. Biochemical characterization of the Helicobacter pylori Cag-type IV secretion system unique component CagU. FEBS Letters 2017-02 | Journal article DOI: 10.1002/1873-3468.12564 Part of ISSN: 0014-5793 PMID: 29572102.
33. Non-heat shock responsive roles of HSF1 in Candida albicans are essential under iron deprivation and drug defense. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2017-02 | Journal article DOI: 10.1016/j.bbamcr.2016.11.021 Part of ISSN: 0167-4889 PMID: 27889440.
34. 2015
35. Biochemical Analysis of CagE: A VirB4 Homologue of Helicobacter pylori Cag-T4SS. PLOS ONE 2015-11 | Journal article DOI: 10.1371/journal.pone.0142606 Part of ISSN: 1932-6203 PMID: 26565397.
36. 2013
37. Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA. PLoS ONE 2013-09-10 | Journal article DOI: 10.1371/jo1. Obtained a good grade with a CGPA of 7.7/9 (2nd Rank) in my pre-PhD coursework from Jawaharlal Nehru University.
RESEARCH PUBLICATIONS:
2023
1. Mycobacterium tuberculosis protein MoxR1 enhances virulence by inhibiting host cell death pathways and disrupting cellular bioenergetics. Virulence. Journal article DOI: 10.1080/21505594.2023.2180230 PMID: 36799069.
2. Regulation of autophagy by SARS‐CoV‐2: The multifunctional contributions of ORF3a. Journal of Medical Virology. Journal article DOI 10.1002/jmv.28959 PMID 37485696.
3. Point‐of‐care optical devices in clinical imaging and screening: A review on the state of the art. Journal of Biophotonics. Journal article DOI 10.1002/jbio.202200386 PMID 36906735.
4. Rv2231c, a unique histidinol phosphate aminotransferase from Mycobacterium tuberculosis, supports virulence by inhibiting host-directed defense. Cellular and Molecular Life Sciences. DOI 10.21203/rs.3.rs-3073689/v1, Under Revision 2.
5. CRISPR‑based diagnostic approaches: Implications for rapid management of future pandemics. Molecular Biology Reports. DOI 10.3892/mmr.2023.13005 PMID 37144477.
6. Multipronged regulation of autophagy and apoptosis: Emerging role of TRIM proteins. Cellular and Molecular Biology letters. Journal article. Accepted.
7. PRKAA2, MTOR, and TFEB in the regulation of lysosomal damage response and autophagy. Journal of Molecular Medicine. Journal article. Under revision 2.
8. Activation of the lysosomal damage response and selective autophagy: The coordinated actions of galectins, TRIM proteins, and CGAS-STING1 in providing immunity against Mycobacterium tuberculosis. Critical Reviews in Microbiology. Journal Article. Under revision 2.
9. Functional Interplay of PE18 and PPE26 Proteins of Mycobacterium tuberculosis: Impact on TH1 Immune Response and Endosome-Phagosome Maturation. Microbes and Infection. Journal article. Under review.
10. 2022
11. 10. COVID-19 and tuberculosis: the double whammy of respiratory pathogens. European Respiratory Review. Journal article DOI: 10.1183/16000617.0264-2021 PMID: 35418488.
12. The exploitation of host autophagy and ubiquitin machinery by Mycobacterium tuberculosis in shaping immune responses and host defense during infection. Autophagy 2022-01 | Journal article DOI: 10.1080/15548627.2021.2021495 PMID: 35000542.
13. ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator. International journal f medical microbiology: IJMM 2021-12 | Journal article DOI: 10.1016/j.ijmm.2021.151544 PMID: 34922100.
14. 2021
15. Mycobacterium tuberculosis Protein PE6 (Rv0335c), a Novel TLR4 Agonist, Evokes an Inflammatory Response and Modulates the Cell Death Pathways in Macrophages to Enhance Intracellular Survival. Frontiers in immunology 2021-07 | Journal article DOI: 10.3389/fimmu.2021.696491 PMID: 34322125.
16. Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic Repurposing, and Immune Modulation. Frontiers in immunology 2021-03 | Journal article DOI: 10.3389/fimmu.2021.636644 PMID: 33746976. Teleological cooption of Mycobacterium tuberculosis PE/PPE proteins as porins: Role in molecular immigration and emigration. International journal of medical microbiology: IJMM 2021-03 | Journal article DOI: 10.1016/j.ijmm.2021.151495 PMID: 33730677.
17. Emerging Transcriptomic Approaches to Decipher Mycobacterial Complexities. Integrated Omics Approaches to Infectious Diseases 2021 | Book chapter DOI: 10.1007/978-981-16-0691-5_7 Part of ISBN: 9789811606908 Part of ISBN: 9789811606915.
18. Exploration of the Mycobacterial Proteome in the Pathogenesis of TB: A Perspective. Integrated Omics Approaches to Infectious Diseases 2021 | Book chapter DOI: 10.1007/978-981-16-0691-5_17 Part of ISBN: 9789811606908 Part of ISBN: 9789811606915.
19. Post-translational modifications in tuberculosis: ubiquitination paradox. Autophagy 2020-11 | Journal article DOI: 10.1080/15548627.2020.1850009 PMID: 33190592.
20. 2020
21. Polypharmacological Drug Repurposing Approach to Target Mycobacterium Tuberculosis. Proceedings of International Conference on Drug Discovery (ICDD) 2020-10-02 | Conference paper.
22. ESX5 associated PE proteins of Mycobacterium tuberculosis drives Th1 type polarization: an immunomodulatory paradigm. The Journal of Immunology 2020 | Journal article WOSUID: WOS:000589972400477.
23. 2019
24. CagW, a VirB6homolog interacts with Cag-type IV secretion system substrate CagA in Helicobacter pylori. Biochemical and biophysical research communications 2019-06 | Journal article DOI: 10.1016/j.bbrc.2019.06.013 PMID: 31182283.
25. Intrinsically Disordered Regions in PE/PPE Protein Family of Mycobacterium tuberculosis: Moonlighting Function. Mycobacterium Tuberculosis: Molecular Infection Biology, Pathogenesis, Diagnostics and New Interventions 2019 | Book chapter DOI: 10.1007/978-981-32-9413-4_9 Part of ISBN: 9789813294127 Part of ISBN: 9789813294134.
26. Mycobacterium Tuberculosis: Molecular Infection Biology, Pathogenesis, Diagnostics and New Interventions 2019 I Book DOI: 10.1007/978-981-32-9413-4 Part of ISBN: 9789813294127 Part of ISBN: 9789813294134 and 978-981-32-9415-8.
27. 2018
28. Identification of genome-wide binding sites of heat shock factor 1, Hsf1, under basal conditions in the human pathogenic yeast, Candida albicans. AMB Express 2018-07 | Journal article DOI: 10.1186/s13568-018-0647-7 Part of ISSN: 2191-0855. PMID: 30014253.
29. 2017
30. Analyzing the role of CagV, a VirB8 homolog of the type IV secretion system of Helicobacter pylori. FEBS Open Bio 2017-05 | Journal article DOI: 10.1002/2211-5463.12225 Part of ISSN: 2211-5463 PMID: 28680806.
31. The global regulator Ncb2 escapes from the core promoter and impacts transcription in response to drug stress in Candida albicans. Scientific Reports 2017-04-06 | Journal article DOI: 10.1038/srep46084 Part of ISSN: 2045-2322 PMID: 28383050.
32. Biochemical characterization of the Helicobacter pylori Cag-type IV secretion system unique component CagU. FEBS Letters 2017-02 | Journal article DOI: 10.1002/1873-3468.12564 Part of ISSN: 0014-5793 PMID: 29572102.
33. Non-heat shock responsive roles of HSF1 in Candida albicans are essential under iron deprivation and drug defense. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2017-02 | Journal article DOI: 10.1016/j.bbamcr.2016.11.021 Part of ISSN: 0167-4889 PMID: 27889440.
34. 2015
35. Biochemical Analysis of CagE: A VirB4 Homologue of Helicobacter pylori Cag-T4SS. PLOS ONE 2015-11 | Journal article DOI: 10.1371/journal.pone.0142606 Part of ISSN: 1932-6203 PMID: 26565397.
36. 2013
37. Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA. PLoS ONE 2013-09-10 | Journal article DOI: 10.1371/journal.pone.0074620 Part of ISSN: 1932-6203 PMID: 24040297.
PROJECTS:1 “Biochemical Characterization of Outer and Inner Membrane Associated Components of Cag-T4SS of Helicobacter pylori” under the supervision of Prof. Gauranga Mukhopadhyay, Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi-110067, India. August 2008-July 2013 2
“Studies on the Bioremediation Potential of Aspergillus niger” under the supervision of Prof. M.Z Abedin, School of Biotechnology, Jamia Hamdard, New Delhi-110062, India.
Jan 2008 - June 2008 3 “Maintenance of MCF-7 and HT-29 Mammalian Cell Lines” under the supervision of Dr. Manu Jaggi, Dabur Research Foundation Ghaziabad, U.P-201010, India May 2007-June 2007 4“Immuno Modulation Potential of Khameera Marwareed”under the supervision of Prof. M.A Baig, School of Biochemistry, Jamia Hamdard, New Delhi-110062, India.
May 2005- July 2005 5 HIGHLIGHTS /EXPERIMENTA), LLS:acterium tuberculosis detection method” under the supervision of Dr. Ravi Krishnan Elangovan, Department of Biochemical Engineering and Biotechnology (DBEB), IIT Delhi, New Delhi-110016, India. May 2017-August 2017 HIGHLIGHTS /EXPERIMENTAL), LLS:ü Throughout my academic and research trajectories, I have developed a robust skill set in molecular biology, molecular genetics, and protein purification techniques. My proficiency extends to fundamental procedures, such as plasmid isolation, genomic DNA isolation, total RNA isolation, cDNA preparation, PCR, cloning, ligation, transformation, electroporation, transfection, and restriction digestion, with a specific focus on Mycobacterium tuberculosis (MTB). Additionally, I am well versed in advanced techniques including PCR, qRT-PCR, gene analysis, SDS-PAGE, Western blotting, 2D gel electrophoresis, and sequencing.
ü In the realm of molecular genetics, I have demonstrated my expertise in protein purification methodologies, including salt fractionation, gel filtration, and affinity purification of MBP, GST, and His-tagged proteins. My skill set further encompasses enzyme kinetics, western blot analysis, RNA isolation, RT-PCR, site-directed mutagenesis, mammalian cell culture, animal handling, antibody generation (Mice and Rabbit), chromatin immunoprecipitation, Helicobacter pylori culture, mutant generation and complementation, pull-down assays, co-expression, and molecular and cellular immunology.
ü Moreover, I possess hands-on experience in employing fluorescence techniques and utilizing spectroscopic tools such as UV-Vis, fluorimeter, confocal microscopy, AFM, and TEM. Complementing my laboratory skills, I am proficient in various computer applications, including statistical packages, image processors (Adobe Photoshop), Image J, basic bioinformatics tools, and online database search tools. This comprehensive skill set underscores mycmy commitmentommitment to employing cutting-edge methodologies to advance molecular and cellular research.
1 Training program on Flow Cytometry's basics and practical application at Jamia Hamdard. 2019. 2 Presented poster titled "Role of RipA in Inflammation and antimicrobial host defense mediated through autophagy" at RCSB and Jamia Hamdard at the International Conference on Infectious Diseases. 2019. 3
The International Conference on Nanotechnology was held in New Delhi. 2019
4“CagE, an essential Cag-T4SS component, forms a complex with inner and outer membrane proteins and is required for CagA translocation and IL-8 induction”. Biosparks 2016, 14th Annual Research Festival (India), held at Jawaharlal Nehru University, New Delhi, India. 2016
5“The global regulator Ncb2 escapes from the core promoter and impact transcription in response to drug stress in Candida albicans cells” in the 9th International Conference on Yeast Biology, organized by Jadavpur University, Kolkata, India. 2015
6“New Horizon in Basic and Clinical Research” organized by the Society of Young Scientists All India Institute of Medical Sciences (AIIMS), New Delhi 110029, at JLN Auditorium.
16th April 2012. 7“Helicobacter pylori T4SS: CagV an inner-membrane associated protein bridges the inner and outer-membrane core complexes” in the XXXV All India Cell Biology Conference (AICB) and Symposium on Membrane Dynamics and Disease organized by theNational Institute of Science Education and Research (NISER), Institute of Physics Campus, Bhubaneswar, Odisha, India.
16-18th December 2011.REFERENCES:
1.
Prof. Seyed Ehtesham Hasnain, Honorary Professor,
DBEB, Indian Institute of Technology, Delhi (IIT, D) Hauz Khas, New Delhi-110 016, India E-mail: sehiitd@gmail.com, seyedhasnain@gmail.com 2.Dr. Nasreen Z. Ehtesham, Scientist Emeritus,
National Institute of Pathology, Safdarjung Hospital Campus, New Delhi-110029, India E-mail: nzehtesham@gmail.com3.
Prof. Gauranga Mukhopadhyay,
Special Centre for Molecular Medicine,
Jawaharlal Nehru University,
New Delhi, 110067,
E-mail: gmukho1@gmail.com
4.
Prof. Rajendra Prasad,
Amity Institute of Integrative Sciences and Health and Amity Institute of Biotechnology,
Gurgaon,
E-mail: rp47jnu@gmail.com
Languages
English
Beginner
Certification
1. Qualified CSIR- NET JRF December 2008 and June 2009 jointly conducted by Council for Scientific and Industrial Research (CSIR) and University Grant Commission (UGC).
2. Qualified CSIR- NET SRF August 2010 jointly conducted by Council for Scientific and Industrial Research (CSIR) and University Grant Commission (UGC).
3. Qualified DBT-JRF in Category A with all INDIA rank 12 in May 2008 conducted by Department of Biotechnology.
4. Qualified GATE with all INDIA rank 161 and 98.7 percentile in March 2008.
5. Obtained a good grade with a CGPA of 7.7/9 (2nd Rank) in my pre-PhD course work from Jawaharlal Nehru University.
Timeline
Senior Scientist
Quantlase Imaging Laboratory
01.2022 - 12.2023
Research Associate
National Institute of Pathology
03.2021 - 02.2022
National Postdoctoral fellow
National Institute of Pathology
04.2018 - 10.2020
Research Associate
IIT, DELHI
05.2017 - 03.2018
Dr. DS KOTHARI Postdoctoral Fellow
School of Life Sciences
02.2014 - 02.2017
Ph.D. - Biochemical characterization of outer and inner membrane complexes of Helicobacter pylori Cag-T4SS
Special Center for Molecular Medicine
08.2008 - 01.2014
M.Sc. - BIOTECHNOLOGY
Jamia Hamdard
07.2006 - 05.2008
B.Sc - Biotechnology
Jamia Milia Islamia
08.2003 - 05.2006
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